Figure 4

HPTLC fingerprinting (A) under UV254 light where tracks 1–3 represent sample prepared with HRE, UAE and MAE methods (using 50% EtOH); whereas 4–8 tracks represent sample harvested at different stages (Juvenile, Vegetative, Bud stage, Flower stage and Fruit stage, respectively) matched with standard compound-mangiferin (1st spot); (B,C) demonstrate 3-D densitogram and overlay spectra of Swertia chirata test samples compared with standard compound (mangiferin).