Table 3 Analytical characteristics of the method validation for mangiferin.

1

Linearity range (ngspot⁻¹; n = 12^a

200–800

2

Correlation coefficient (r²)

0.987

3

Regression equation

Y = − 608.4 + 9.033X

4

Calculated SD value (CATS software)

6.72

5

^bLimit of detection (LOD) (ng) [3 × SD/S]

25

6

^bLimit of quantitation (LOQ) (ng) [10 × SD/S]

75

7

R_f

0.47

8

λ_{max (nm)}

258

Precision and accuracy

7

Intra-day RSD (%), n = 5

0.437

8

Inter-day RSD (%),n = 5 (day-1/day-2/day-3)

0.437/0.495/0.512

Recovery

9

Amount of mangiferin in leaf samples (μg mg⁻¹) containing highest mangiferin

48.6

10

Amount of mangiferin added in leaf sample (μg mg⁻¹)

20/40/60

11

Amount of mangiferin found (μg)

68.36 ± 0.251/88.4 ± 0.36/108.26 ± 0.35

12

Recovery (%) (mean)

99.99/99.77/99.68 (99.81)

13

RSD (%)

0.36/0.407/0.323