Figure 2 | Scientific Reports

Figure 2

From: P2Y12 receptor blockers are anti-inflammatory drugs inhibiting both circulating monocytes and macrophages including THP-1 cells

Figure 2

Presence of monocyte platelet aggregates in circulating blood of healthy volunteers and patients with CAD and ACS using different monocyte isolation methods. To determine whether MPA are present in all monocytes isolated from whole blood, levels of MPA were assessed using different methods as described in the “Methods” section. (a) Platelets adherent to monocytes were found in small numbers in the blood smears of healthy subjects. The light microscopic image depicts a monocyte with two adhering platelets indicated by arrows. Scale bar = 10 µm. (b) Quantification of MPA in percentage of all monocytes in blood smears demonstrated increasing percentages of monocytes with adherent platelets in healthy volunteers, CAD and patients with acute coronary syndrome. n = 5 per group. (c) Microscopic image of a representative monocyte isolated by Ficoll density gradient centrifugation at 1000× magnification using an inverted microscope. (d) MPA were found in healthy subjects after isolation of monocytes using different isolation methods. n ≥ 3, p-values were calculated using one-way ANOVA.

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