Figure 1
From: NOTUM promotes thermogenic capacity and protects against diet-induced obesity in male mice
NOTUM overexpression protects against diet-induced obesity in mice. (a–k) Male C57BL/6J mice were infected with AAV-GFP or AAV-Notum (n = 12 per group) and fed a HF/HS diet for 14 weeks to induce obesity. (a) Liver NOTUM and GAPDH protein levels as determined by immunoblotting (n = 8/group). The images shown for NOTUM and GAPDH were from different parts of the same blot. Liver NOTUM/GAPDH ratios are shown in the right panel. (b) Plasma NOTUM and APOE levels as determined by immunoblotting. n = 8/group. The images shown for NOTUM and APOE were from different parts of same blot. The plasma NOTUM/APOE ratios are shown in the right panel. (c) Average cumulative food intake per mouse during the first 12 weeks of diet feeding is shown. Body weight (d), fat mass (e), and lean mass (f) of mice during the diet feeding period are shown. n = 12/group. At euthanasia (14 weeks), body weight (g), and weights of eWAT, iWAT, mWAT, rWAT, and iBAT (h) were measured. n = 12/group. (i–k), n = 11 to 12/group: After five-week feeding of the HF/HS diet, mice were individually housed for 48 h for measurements of food consumption (i). Fecal samples were collected for determination of fecal energy content (j). (k) Net energy intake was then calculated (see “Material and methods” section). (l–o) In an independent experiment, AAV-GFP and AAV-Notum infected mice (n = 6/group) were fed a HF/HS diet for 6 weeks before the mice were individually housed in metabolic cages for 3 days for measurement of energy expenditure by indirect calorimetry. Oxygen consumption rate, (l) and (m), and energy expenditure rate (n) and (o) are shown. Symbols: *: p < 0.05, **: p < 0.01, ***: p < 0.001, AAV-GFP versus AAV-Notum as determined by Student’s t-test.
