Figure 3
From: NOTUM promotes thermogenic capacity and protects against diet-induced obesity in male mice
NOTUM overexpression improves glucose tolerance and increases lipolysis capacity. AAV-GFP and AAV-Notum infected mice (n = 11 to 12 mice per group) were maintained on a HF/HS diet for 10 to 12 weeks before the following experiments were performed. (a) IPGTT was performed as described in “Material and methods” section. Blood glucose levels, right, and glucose AUC, left, are shown. (b) ITT was performed as described in “Material and methods”. Blood glucose levels, left, and glucose AUC, right, are shown. (c) ATGL and HSL protein levels in iWAT were determined by immunoblotting (n = 6 per group), left. Relative protein levels of ATGL and HSL, as normalized by GAPDH levels, were shown in right. Images of ATGL, HSL, and GAPDH were obtained from different parts of the same blot. (d, e) β3 adrenergic receptor agonist stimulated lipolysis was performed as described in “Material and methods” section. Plasma free fatty acid (FFA, d) and glycerol (e) levels at time 0 (basal) and 30 min after injection of CL316,243 (CL) are shown (n = 10–12 per group). Symbols: *: p < 0.05, **: p < 0.01, AAV-GFP versus AAV-Notum as determined by Student’s t-test. For (c) and (d), #: p < 0.05 versus time 0 (basal) of the same AAV group.
