Figure 4
From: NOTUM promotes thermogenic capacity and protects against diet-induced obesity in male mice
NOTUM overexpression promotes thermogenic capacity in WATs and iBAT and decreases adipose tissue inflammation and fibrosis. IWAT, eWAT, and iBAT samples from the same mice described in Fig. 1a–i were used for the following assays. (a) Gene expression analysis by qPCR of iWATs are shown. n = 9–12/group. (b) Protein levels of UCP1 and β-ACTIN in the iWAT were determined by immunoblotting (top) and quantification of the UCP1 protein normalized by the β-ACTIN protein is shown at the bottom. n = 4. Images of UCP1 and β-ACTIN were obtained from different parts of same blot. Gene expression analysis by qPCR, (c), CCL2 protein content, (d), and hydroxyproline content, (e), of eWAT are shown. Gene expression analysis (n = 10–12), (f), and UCP1 and β-ACTIN protein levels as determined by immunoblotting, (g), (n = 5) of iBAT are shown. Images of UCP1 and β-ACTIN were obtained from different parts of same blot. Symbols: #: p ≤ 0.09, *: p < 0.05, **: p < 0.01, ***: p < 0.001, AAV-GFP versus AAV-Notum, as determined by Student’s t-test.
