Figure 2 | Scientific Reports

Figure 2

From: Metformin attenuates renal tubulointerstitial fibrosis via upgrading autophagy in the early stage of diabetic nephropathy

Figure 2

Effects of metformin on expression of FN and collagen I in DN kidneys. (a) Collagen I was observed by immunohistochemistry under a microscope. Scale bar represents 50 μm. (b) Expression of FN and collagen I based on immunoblotting and normalized to β-actin. The boxes separate images from different gels, but samples derived from the same experiment and blots were processed in parallel. (c) Statistical analysis of the expression of FN and Collagen I in each group. *P < 0.01 vs. Sham group by ANOVA, #P < 0.01 vs. Sham and 4-week DN group by ANOVA, $P < 0.05 vs. 4-week DN/DN + Met group by ANOVA. 4W: 4-week; 12W: 12-week; Met: metformin. Full-length blots were presented in Supplementary Figures.

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