Figure 5
Effects of metformin on the expression of p-AMPK, LC 3I, LC 3II and FN in HG-stimulated RTECs. (a) Metformin and AICAR upgraded the p-AMPK expression in HG-stimulated RTECs. The boxes indicate images from different gels. (b) Statistical analysis of p-AMPK expression in each group. *P < 0.01 compared with the LG group, #P < 0.01 compared with the HG without metformin group, △P < 0.01 compared with the HG group, $P < 0.01 compared with HG + Met group, &P < 0.01 compared with HG + AICAR group. (c) Metformin positively regulated the ratio of LC 3II/I and downgraded the expression of FN, while Baf A1 negatively regulated the ratio of LC 3II/I and upgraded the expression of FN. The boxes indicate images from different gels, but the samples derived from the same experiment and blots were processed in parallel. (d) Statistical analysis of FN expression and the ratio of LC 3II/I in each group. *P < 0.01 compared with the HG without metformin and Baf A1 group, #P < 0.01 compared with the HG with Baf A1 but without metformin group. Full-length blots were presented in Supplementary Figures.
