Figure 1

(A1) Crystal structure of Adalimumab heavy chain Fab fragment. (A2) Sequence map of Adalimumab heavy chain Fab fragment. Relevant candidate immunogenic peptide epitopes are reported. (B1) Adalimumab light chain Fab fragment. (B2) Sequence map. Underlined peptide fragments correspond to the same fragments reported in (A1). Different colors refer to the epitope identification methods used. Yellow: TNF-α contact points: TNF-α paratope was identified by crystal structure analysis of the antigen–antibody complex. Blue: epitope mapping by patient serum ELISA. Green: T-cell epitope mapping. Red: CDRs identified according to Kabat rules.