Figure 2

Nodulation properties of V. radiata cv. KPS1 inoculated with B. diazoefficiens strains. The roots were imaged at 11 (A) and 15 (B) dpi. (C) Nodule primordia per centimetre of root tissue and (D) numbers of young nodules per plant observed at 7, 11 and 15 dpi. (E) Nodule morphology and propidium iodide (PI)/SYTO9 staining of sectioned nodules. The data shown are the means of at least 6 plants at 7 and 15 dpi and of 18 plants at 11 dpi. The basal regions of lateral roots were sampled (3 samples per plant, n = 18) for microscopy analyses. Scale bars: 1 cm, entire roots; and 2 mm, nodule primordia and young nodules. “*”P < 0.05 and “**”P < 0.01 according to Student’s t-test.