Figure 2 | Scientific Reports

Figure 2

From: Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci

Figure 2

Assessment of virulence of C. gallinacea in embryonated eggs. (A) The 50% egg infective dose 50 (EID50) of C. gallinacea NL_G47, NL_F725 and C. psittaci NL_Borg based on IFT of the yolk sac. The difference between EID50 of NL_G47 and NL_Borg was significantly different (*, P < 0.05, Wilcoxon–Mann–Whitney test). For C. psittaci NL_Borg the 50% lethal dose (LD50) was also calculated. The median EID50 or LD50 of the experiments is indicated with a bar. (B–D) Depict the cumulative results of the separate titration experiments per Chlamydia strain. Per dilution, the percentage of eggs that was positive for Chlamydia in the immunofluorescence test (IFT) with mortality, IFT positive without mortality and IFT negative are shown. The total number of eggs per dilution are presented at the top of every bar. These data are also included in Supplementary Table S1. The figure was created in GraphPad Prism 9.0.0.

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