Figure 1 | Scientific Reports

Figure 1

From: EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway

Figure 1

EMID1 expression in mouse cell lines. (A) Emid1 mRNA expression in vitro measured with real-time qRT-PCR. Error bars, ± standard deviation (SD) (n = 3). (B) Effect of stable transfection of the Emid1 expression vector on 66–4 cells. Overexpression of EMID1 was confirmed with real-time qRT-PCR (upper left), western blotting (upper right), and immunofluorescence (bottom). Error bars, ± SD (n = 3). *P < 0.05. Scale bar, 100 μm. (C) Knockdown effect of siRNAs for Emid1 on 66HM cells. Relative mRNA levels of the transfectants with specific siRNAs targeting three different sequences (siE1, siE2, and siE3) were measured with real-time qRT-PCR. Error bars, ± SD (n = 3). *P < 0.05.

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