Figure 2
Effect of EMID1 expression on cell properties in vitro. (A) Cell proliferation analysis using the XTT assay. 66–4 cells stably overexpressing EMID1 and 66HM cells transfected with siRNA at 48 h were measured. Error bars, ± SD (n = 3). **P < 0.01. (B) Cell cycle analysis using flow cytometry. 66HM cells transfected with siE2 at 24 h were analyzed. Error bars, ± SEM (n = 3). *P < 0.05. (C) Cell migration analysis using the wound healing assay. The speed of wound closure for 24 h of both overexpressing and knockdown cells was evaluated (upper). Error bars, ± SD (n = 3). **P < 0.01. Phase contrast images of migrating cells (bottom). Scale bar, 500 μm. (D) Cell invasion analysis using the biocoat matrigel chamber. After incubation of 66–4 and 66HM transfectants for 8 and 12 h, respectively, migrating cells were counted (upper). Error bars, ± SD (n = 3). *P < 0.05. Representative image of invading cells stained with hematoxylin (bottom). Scale bar, 100 μm.
