Figure 3
Expression and subcellular localization of EMID1 protein. (A) Western blots in non-reducing conditions of cell fractions of 66–4 cells transfected with the Emid1 expression vector. (B) Immunofluorescent image of in vitro localization of EMID1 protein overexpressed in 66–4 cells. EMID1 protein is expressed in the cytoplasm (left) and deposited on the dish surface with brush-like structures, which are seen following cell detachment with Triton-X treatment (right). Scale bar, 50 μm. (C) Representative immunohistochemical image of EMID1 protein expression in vivo tumor tissue of EMID1-overexpressing 66–4 cells and 66HM cells orthotopically inoculated into the mammary fat pad of syngeneic mice. EMID1 is linearly localized around the tumor nests of both 66HM cells (left) and 66–4 cells transfected with Emid1 expression vector (right). Scale bar, 100 μm.
