Figure 4
Effects of EMID1 expression on cell morphology and adhesion in vitro. (A) Morphological changes associated with EMID1 overexpression and EMID1 knockdown. Phase contrast images in sparse conditions (a, d, h, k). Scale bar, 100 μm. Filamentous actin formation stained with phalloidin conjugated with rhodamine (b, e, i, l). Scale bar, 50 μm. Phase contrast images (c, f, j, m) and immunofluorescence with EMID1 antibody (g) in confluent conditions. Scale bar, 100 μm. (B) Effect of EMID1 deposition on cell-substrate adhesion. A schematic diagram of the following experiment (a). The image was drawn using Microsoft PowerPoint 2016, https://www.microsoft.com/en-US/download/details.aspx?id=53373. The number of cells adhering to the dish in which EMID1-overexpressing 66–4 cells had been cultured was reduced compared to control cells (b). Phase contrast images of adherent cells on the dishes without and with EMID1 protein deposition (c). Scale bar, 50 μm.
