Figure 9
Phenazine-1-carboxylic acid production by survivor P. putida and potential contribution to predator avoidance trait. (A) Principal component analysis (PCA) score plot of untargeted mass spectrometry data from crude extracts of parent P. putida (green), survivor P. putida (blue), and media extracts with no bacteria added as a control (red). XCMS v3.7.1 was used to render the PCA score plot. (B) Extracted ion chromatograph (224.9–225.1 m/z) depicting presence of phenazine-1-carboxylic acid in extracts from survivor P. putida (n = 3; magenta, yellow, cyan) and absence in extracts from parent P. putida replicates (n = 3; red, blue, green (baseline)). Chromatograph rendered with MZmine v2.37. (C) Comparison of swarm diameters for C. ferrugineus cultivated on filter-sterilized supernatants from parent P. putida and survivor P. putida cultivation broths (n = 3; p = 0.039). Statistical significance calculated from an unpaired t test with Welch’s correction.
