Table 1 Sensitivity and specificity of reverse transcription (RT) polymerase chain reactions (PCR) targeting the M segment of the SFTS virus (single-round PCR-M), RT nested PCR targeting the M-segment of the SFTS virus (NPCR-M), RT-nested PCR targeting the S-segment of the SFTS virus (NPCR-S), and real-time RT PCR targeting the S-segment of the SFTS virus (QPCR-S) conducted in this study for their clinical evaluation of 36 samples from severe fever with thrombocytopenia (SFTS) patient and 85 samples from non-SFTS infectious disease cases.
PCR assay | Single round PCR(M) | N-PCR(M) | N-PCR(S) | q-PCR | ||||
|---|---|---|---|---|---|---|---|---|
Case | Control | Case | Control | Case | Control | Case | Control | |
PCR positive | 23 | 0 | 36 | 0 | 34 | 0 | 34 | 0 |
PCR negative | 13 | 85 | 0 | 85 | 2 | 85 | 2 | 85 |
Total | 36 | 85 | 36 | 85 | 36 | 85 | 36 | 85 |
Sensitivity, % (95% CI) | 63.9 (46.2–79.2) | 100 (90.3–100) | 94.4 (81.3–99.3) | 94.4 (81.3–99.3) | ||||
Specificity, % (95% CI) | 100 (95.7–100) | 100 (95.8–100) | 100 (95.8–100 | 100 (95.8–100) | ||||
PPV, % (95% CI) | 100 | 100 | 100 | 100 | ||||
NPV, % (95% CI) | 86.7 (80.9–91.0) | 100 | 97.7 (91.7–99.4) | 97.7 (91.7–99.4) | ||||
AUC (p < 0.001) | 0.819 (0.739–0.883) | 1.000 (0.970–1.000) | 0.972 (0.925–0.994) | 0.972 (0.925–0.994) | ||||
