Table 2 Comparison of QPCR-S (real-time reverse transcription-RT polymerase chain reaction-PCR targeting the S-segment of the SFTS virus) negative samples (Ct > 42) with NPCR-M (RT nested PCR targeting the M-segment of the SFTS virus) results for blood samples tested from severe fever with thrombocytopenia syndrome (SFTS) patients in the study.
Cycle threshold (Ct) | Day after symptom onset | QPCR-S | N-PCR-M | ||
|---|---|---|---|---|---|
No. of negative samples | (Ct) | Result | No. of positive samples | ||
39 < Ct < 42 | – | 18 | – | – | 16/18 |
3 | 40.11 | Positive | |||
5 | 39.59 | Positive | |||
7 | 39.35 | Positive | |||
8 | 40.02 | Positive | |||
8 | 41.5 | Negative | |||
10 | 41.1 | Positive | |||
10 | 39.71 | Positive | |||
10 | 39.5 | Positive | |||
11 | 39.28 | Positive | |||
12 | 40.76 | Positive | |||
13 | 39.68 | Positive | |||
14 | 39.53 | Positive | |||
18 | 39.63 | Positive | |||
21 | 40.38 | Positive | |||
23 | 39.34 | Negative | |||
23 | 40.71 | Positive | |||
25 | 41.98 | Positive | |||
39 | 40.62 | Positive | |||
42 < Ct | – | 5 | – | – | 1/5 |
7 | 42.07 | Negative | |||
9 | 43.15 | Negative | |||
11 | 42.03 | Negative | |||
15 | 43.96 | Positive | |||
26 | 42.99 | Negative | |||
Undetermined | – | 12 | – | – | 0/12 |
