Table 3 Oligonucleotide primers, probes, product base pair (bp) size and conditions used for polymerase chain reactions (PCR) conducted in this study for the detection of severe fever with thrombocytopenia syndrome (SFTS).
PCR assay | Primers and probe name (sequence) | PCR conditions (35 cycles) | Product size (bp) | References | ||
|---|---|---|---|---|---|---|
Denaturing (°C/sec) | Annealing (°C/sec) | Extension (°C/sec) | ||||
SFTS RT-N-PCR (M segment) | SFTS-M 1st-F | 95/20 | 58/40 | 72/30 | 510 | This study |
(5′-TCATCCTGACYTATTYTGCAATWG-3′) | ||||||
(Eexternal primer) | SFTS-M 1st-R | |||||
(5′- TAAGTYACACTCACACCCTTGAA-3′) | ||||||
SFTS RT-PCR | SFTS-F | 95/20 | 58/40 | 72/30 | 560 | Yun et al.12 |
(5′-GATGAGATGGTCCATGCTGATTCT-3′) | ||||||
(Internal primer) | SFTS-R | |||||
(5′-CTCATGGGGTGGAATGTCCTCAC-3′) | ||||||
SFTS RT-N-PCR (S segment) | SFTS-S-NP-2F | 94/20 | 53/20 | 72/30 | 461 | Hwang et al.13 |
(5′-CATCATTGTCTTTGCCCTGA-3′) | ||||||
(External primer) | SFTS-S-NP-2R | |||||
(5′-AGAAGACAGAGTTCACAGCA-3′) | ||||||
SFTS RT-PCR | SFTS-S-N2F | 94/20 | 57/20 | 72/30 | 346 | Hwang et al.13 |
(5′-AAYAAGATCGTCAAGGCATCA-3′) | ||||||
(Internal primer) | SFTS-S-N2R (5′-TAGTCTTGGTGAAGGCATCTT-3′) | |||||
SFTS-real time (S segment) | SFTS-SQ-F | 95/05 | 95/10 | 51/10 | – | Heo, et al.14 |
(5′-ACCTCTTTGACCCTGAGTTWGACA-3′) | ||||||
SFTS-SQ-R | ||||||
(5′-CTGAAGGAGACAGGTGGAGATGA-3′) | ||||||
SFTS-SQ-P | ||||||
(FAM-5′-TGCCTTGACGATCTTA-NFQ-MGB -3′) | ||||||
