Figure 3 | Scientific Reports

Figure 3

From: Thermostable adenosine 5′-monophosphate phosphorylase from Thermococcus kodakarensis forms catalytically active inclusion bodies

Figure 3

Ni-NTA purification of His-tagged TKAMPpase and its thermal stability. (a,b) 12% SDS-polyacrylamide gels showing the samples taken during the purification of TKAMPpase. Loaded sample (LS), flowthrough (FT), wash fractions (W1, W2, W3) and elution fractions (E1–E6). The FT from column 1 was loaded consecutively to column 2. (c) Heat treatment of the purified TkAMPpase by incubation at 60 °C and 80 °C for 1 h and 24 h. A sample was taken before incubation (0 h).

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