Figure 7

Effect of compounds on tau protein levels. Representative western blot images (A) from one of 3–4 replicate sets collected per compound. Full-length blots are presented in Supplementary Fig. S12. (B) Quantitation of western blot band intensities showing the effect of 20 h treatment with CHIR-99021, enzastaurin or ruboxistaurin on TAU5 and Tau-pS396 in both control and Tau-A152T iNgn2 neurons. Each bar represents mean ± SEM of at least 3 biological replicates; dots represent individual values. TAU5 and Tau-pS396 band intensities were normalized to GAPDH band intensities to control for variation in sample loading. To account for blot to blot variability in signal intensity, band intensities for drug treatment lanes were normalized to the DMSO band intensity of each cell line in each blot. The dotted line represents the GAPDH-normalized signal intensity of DMSO, set to 1. One-way ANOVA, Dunnett’s post hoc test, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 compared to DMSO.