Table 1 Primers used in real-time RT-PCR.
From: LOXL2 attenuates osteoarthritis through inactivating Integrin/FAK signaling
Gene | Primer sequences (5′–3′) |
|---|---|
GAPDH | Fwd: 5′-AAAGGCATCTTGGGCTACACCG-3′ |
Rev: 5′-ATGAGGTCCACCACCCTGTTG-3′ | |
LOLX2 | Fwd: 5′-CGATTGCCACCTCCTTGCTA-3′ |
Rev: 5′-CCAGAGCCCTGCCCCTAA-3′ | |
Collagen II | Fwd: 5′-AGAGCGGAGACTACTGGATTG-3′ |
Rev: 5′-TCTGGACGTTAGCGGTGTT-3′ | |
TNF-a | Fwd: 5′-CCACGCTCTTCTGTCTACTG-3′ |
Rev: 5′-GCTACGGGCTTGTCACTCGA-3′ | |
Integrin β1 | Fwd: 5′-AATTCAAGAGGGCTGAAGACTAC-3′ |
Rev: 5′-TGTCAGTAAGACTAAGCACG-3′ | |
FAK | Fwd: 5′-CCAAGTTCGAGTACTAAGACTCACC-3′ |
Rev: 5′-AAATCCATAGCAGGCCACGTGC-3′ |
