Figure 2
From: Rational design of heterodimeric receptors capable of activating target signaling molecules
Comparison of the single transductants and double transductants of the FKBP and FRB chimeras. The symbols Δ, 3, and 5 mean no motif, the STAT3-binding motif, and the STAT5-binding motif, respectively. Parental Ba/F3 and the transduced cells were unstimulated (−) or stimulated with 50 nM AP21967 (+). Phospho-STAT3, whole STAT3, phospho-STAT5, whole STAT5, HA tag (FKBP chimera), V5 tag (FRB chimera), Myc tag (both FKBP and FRB chimeras; upper and lower bands, respectively), and GAPDH (loading control) were detected using corresponding primary antibodies. Full-length blots are presented in Supplementary Fig. 8.
