Table 3 Primers used for related experiments in this study.
Primer purpose | Primer name | Primer sequence (5′ → 3′) |
|---|---|---|
5′ RACE | MK-5(1) | CGCAGATGAACCCAATCCAGAAC |
MK-5(2) | CCTCGGGAATGTTCTGCTCTTCA | |
PMK-5(1) | TGTCAGAGGGAGCCCACGTGCCT | |
PMK-5(2) | AAGAGTACTGCACCGCTTGTTCT | |
3′ RACE | MK-3(1) | CGACAACACGGTCAGCACATA |
MK-3(2) | AATGAATCAGGGGTTGCTCCA | |
PMK-3(1) | ATGGCTGTAGTTGCTTCTGCTCC | |
PMK-3(2) | GTGAAACTAACATCTCCTCAGCTC | |
ORF | MK-O(F) | ATGGAGGTGAGGGCTCGAGCTC |
MK-O(R) | TGAAGAACCACCGAGACAAATCT | |
PMK-O(F) | ATGGCTGTAGTTGCTTCTGCTCC | |
PMK-O(R) | TCCAATGTGAACTGAAGAAACAG | |
qRT-PCR | q-MK-F | GCTTCCTCTAGGTTCTGGATTG |
q-MK-R | CTGCGGTCCAAAGTTACTGTAT | |
q-PMK-F | GACTGGCGGTTACCTCATTT | |
q-PMK-R | GCTTGACATCATCGTGAATTGG | |
Functional complementation | pYES2-MK-F | CCGGAATTCGAATGGAGGTGAGGGCTCGAGCTC |
pYES2-MK-R | TAAAGCGGCCGCGTGAAGAACCACCGAGACAAATCT | |
pYES2-PMK-F | CCGGAATTCGAATGGCTGTAGTTGCTTCTGCTCC | |
pYES2-PMK-R | TAAAGCGGCCGCGTCCAATGTGAACTGCTGAAG | |
Subcellular localization | YFP-MK-F | CCGGAATTCATGGAGGTGAGGGCTCGAGCTC |
YFP-MK-R | TCCCCCGGGTGAAGAACCACCGAGACAAATCT | |
YFP-PMK-F | CCGGAATTCATGGCTGTAGTTGCTTCTGCTCC | |
YFP-PMK-R | CGCGGATCCATCCAATGTGAACTGAAGAAACA |
