Figure 4 | Scientific Reports

Figure 4

From: The retinoid X receptor α modulator K-80003 suppresses inflammatory and catabolic responses in a rat model of osteoarthritis

Figure 4

K-80003 enhances ERα signaling by dissociation of RXRα-ERα interaction. (A) HEK293T cells transfected with the RXRα and ERα plasmids were treated with K-80003 (1 μM, 10 μM) for 2 h and then analyzed by co-IP assays. (B) Primarily cultured rat chondrocytes were treated with K-80003 (1 μM, 5 μM, 25 μM) and analyzed by co-IP assays using anti-RXRα or anti-ERα antibody. Rat primary chondrocytes were transfected with RXRα SiRNA (0.5 μM), or ERα SiRNA (0.5 μM), or control siRNA (0.5 μM) with Lipofectamine 3000 for 24 h, following by incubation with vehicle, or K-80003 (5 μM, 10 μM) or 17β-estradiol (10 nM) for 30 min. Cells were then treated with IL-1β (10 ng/mL) for 48 h before analyzed expression of ERα by RT-PCR (C) and western-blot (D). (E) Immunofluorescence analyses and quantification of ERα in the tibia medial compartment of animals. ***P < 0.001; n = 5.

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