Figure 2
From: The chaperone HSPB1 prepares protein aggregates for resolubilization by HSP70
HSPB1-3D regulates the size and morphology of aggregates. (a–c) Representative negative stained electron micrographs of (a) native HSPB1 and HSPB1-3D; and of (b) luciferase or (c) LDH before and after incubation for 15 min at 45 °C or 55 °C, respectively. The aggregates in the presence of 20 µM HSPB1 or HSPB1-3D were prepared with 2 µM luciferase or LDH. Luciferase alone could not be detected at 2 µM, so preHS and substrate alone samples were prepared also using 20 µM luciferase or LDH. All scale bars represent 200 nm.
