Figure 4
From: The chaperone HSPB1 prepares protein aggregates for resolubilization by HSP70
HSPB1-3D changes oligomeric state during disaggregation. (a–c) The elution patterns of HSPB1-3D (a) before the heat shock or in the disaggregation reactions with (b) luciferase or (c) LDH as in Fig. 3c–f, were assessed by SEC and immunoblot (IB). (d, e) Quantification of HSPB1-3D in the reactions with (d) luciferase and (e) LDH from B and C are shown as percentage of the total amount of immunoblot signal. (f) HSPB1-3D in the absence of substrates was submitted to heat shock at 45 ℃, transferred to reaction buffer with (+ Chap) or without (− Chap) chaperones and analyzed by SEC and immunoblot. (g) Quantification of HSPB1-3D elution pattern in f. Error bars show standard deviations, n ≥ 3.
