Figure 6

HSPB1-3D-GxG assists disaggregation of LDH but not luciferase. (a, b) Disaggregation of (a) luciferase and (b) LDH aggregates prepared with or without HSPB1-3D-GxG was assessed through the measurement of substrate enzymatic activity recovery as in Fig. 5. Aggregates prepared with HSPB1-3D were used as positive controls and reactions in the absence of chaperones were used as negative controls. Native substrate activity was set as 100% activity. (c, d) The effect of HSPB1-3D-GxG in LDH disaggregation. (c) LDH and (d) HSPB1-3D-GxG elution patterns at the beginning (0 h) and end (3 h) of reactions with (+ Chap) or without (− Chap) chaperones were determined by SEC and immunoblot (IB) as in Fig. 3. (e, f) Quantification of (e) LDH or (f) HSPB1-3D-GxG from C and D in eluted fractions are shown as percentage of the total amount of immunoblot signal. Error bars show standard deviations, n ≥ 3.