Figure 6

Expression of TLRs (a) and cytokines (b) in chMoDCs at 6 and 24 h post-infection with S. Gallinarum (SG) and S. Typhimurium (ST) (multiplicity of infection 10). The mRNA expression for pro- and anti-inflammatory cytokines, related key chemokines, and toll like receptors were determined by real time-quantitative polymerase chain reaction (RT-qPCR). The mock-infected cells were used as control for calculating the fold change of differential gene expressions in the infected cells. (a) Data were analysed by two-way repeat measures ANOVA for S. Typhimurium and S. Gallinarum at 6 and 24 h post-infection. LPS served as positive control but not included in the analysis. Data for negative control is not shown; (b) Data were analysed by two-way repeat measures ANOVA for S. Typhimurium and S. Gallinarum at 6 and 24 h post-infection. Data for negative control served as calibrator group to calculate the fold change differences. Values shown are mean ± SEM of three independent experiments (*P < 0.05, **P < 0.001).