Figure 1
From: Doxycycline promotes proteasome fitness in the central nervous system
Doxycycline reduces accumulation of CNS K48Ub-linked proteins in a region and sex specific manner. (A) Schematic of the experimental design, (B) Western blot of K48Ub-linked proteins in the spinal cords of wild-type female mice (n = 4) that were untreated or treated with doxycycline (Dox) alone or with heat shock (HS). Loading was determined using actin. (C) Quantification of (B). (D) Western blot of K48Ub-linked proteins in the spinal cords of wild-type male mice (n = 4) that were untreated or treated with doxycycline (Dox) alone or with heat shock (HS). Loading was determined using actin. (E) Quantification of (D). (F) Western blot of K48Ub-linked proteins in the brain of wild-type female mice (n = 4) that were untreated or treated with doxycycline (Dox) alone or with heat shock (HS). Loading was determined using actin. (G) Quantification of (F). (H) Western blot of K48Ub-linked proteins in the brain of wild-type male mice (n = 4) that were untreated or treated with doxycycline (Dox) alone or with heat shock (HS). Loading was determined using actin. (I) Quantification of (H). (J) Western blot of K48Ub-linked proteins in the cerebellum of wild-type female mice (n = 4) that were untreated or treated with doxycycline (Dox) alone or with heat shock (HS). Loading was determined using actin. (K) Quantification of (J). (L) Western blot of K48Ub-linked proteins in the cerebellum of wild-type male mice (n = 4) that were untreated or treated with doxycycline (Dox) alone or with heat shock (HS). Loading was determined using actin. (M) Quantification of L. * or **, indicates p < 0.05 or p < 0.005 by student’s t-test. For western blots in all panels, samples were run on the same gel in the indicated order with an empty well between groups to facilitate visualization and none of the images were cropped.
