Figure 5
From: Doxycycline promotes proteasome fitness in the central nervous system
Doxycycline activates the proteasome in yeast in a Mms19 dependent manner. (A) Activity of the proteasome in wild-type yeast cells with and without doxycycline (n = 3 biological replicates with 2–3 technical replicates per culture) after 90 min of treatment values were normalized to the untreated control in both groups. (B) Activity of the proteasome in Mms19 knockout yeast cells (mms19Δ) with and without doxycycline. (n = 3 biological replicates with 2–3 technical replicates per culture). (C) Western blot of K48Ub-linked proteins in wild-type and mms19Δ yeast cells with and without doxycycline (n = 3 biological replicates). The samples were run on the same gel in the indicated order. (D) Quantification of (C). (E) Model of the proposed differential route of activation of the proteasome in yeast or male and female spinal cords. * or **, indicates p < 0.05 or p < 0.005 by student’s t-test.
