Figure 1
From: First identification of ITM2B interactome in the human retina
Experimental design. ITM2B is composed of several domains: CD: cytosolic domain, TMD: transmembrane domain, BRICHOS, Bri23 and two linker domains (in light grey). Two distinct anti-ITM2B antibodies were used for immunoprecipitation. The first antibody is a rabbit polyclonal antibody raised against the 15–264 aminoacidic region of ITM2B and the second antibody is a mouse monoclonal antibody raised against the N-terminal 1–54 aminoacidic region of the protein. A third unspecific IgG mouse antibody was used as negative control. After ITM2B immunoprecipitation, protein complexes were digested with trypsin and peptide mixtures were analyzed by LC–MS/MS. A label-free quantitative proteomic analysis was then performed to identify and quantify ITM2B partners.
