Table 1 List of primary and secondary antibodies used for immunoprecipitation and Western blot.
From: First identification of ITM2B interactome in the human retina
Antigen | Species | Dilution | Source |
|---|---|---|---|
ITM2B | Mouse | IP: 6 µg; WB: 1/500 | Santa Cruz (sc-374362) |
Rabbit | WB: 1/1000 | Thermo Fisher Scientific (PA531441) | |
APP | Rabbit | IP: 5 µg; WB: 1/1000 | Merck (HPA001462) |
Mouse | WB: 1/500 | Merck (MAB348) | |
APLP2 | Mouse | IP: 10 µg | Merck (SAB1410669) |
WB: 1/500 | |||
COX5B | Rabbit | IP: 2 µg; WB: 1/500 | Thermo Fisher Scientific (PA5-96189) |
Myc | Mouse | WB: 1/500 | Roche (11667149001) |
γ Tubulin | Mouse | WB: 1/10,000 | Merck (T6557) |
IgG isotype control | Mouse | According to the specific antibody used | Cell Signaling (5415) |
IgG isotype control | Rabbit | According to the specific antibody used | Cell Signaling (3900) |
Mouse HRP | Donkey | WB: 1/20,000 | Jackson ImmunoResearch (715-035-150) |
Rabbit HRP | Goat | WB: 1/20,000 | Jackson ImmunoResearch (111-035-003) |
