Figure 1 | Scientific Reports

Figure 1

From: Identification of new target proteins of a Urotensin-II receptor antagonist using transcriptome-based drug repositioning approach

Figure 1

Overall scheme of this study. (a) Samples were prepared from four cell lines treated with KR-37524 or dimethyl sulfoxide (DMSO). (b) Microarray data of KR-37524 induced transcriptome changes were generated from this sample, and the fold change values were calculated to measure DEGs for the 978 landmark genes. (c) The gene expression patterns were compared with the LINCS L1000 data to find similar perturbagen signatures, and (d) the top three candidate target proteins were selected using the Zhang score for each cell line. (e) Finally, experimental validation was performed to confirm the affection of KR-37524 on the candidate target protein. The protein structure was drawn by Chimera software34 (www.cgl.ucsf.edu/chimera).

Back to article page