Figure 3 | Scientific Reports

Figure 3

From: Development of multiplex digital PCR assays for the detection of PIK3CA mutations in the plasma of metastatic breast cancer patients

Figure 3

Evaluation of the sensitivity of the PIK3CA assays. DNA mixes were prepared through serial dilutions of MUT gBlocks in a constant WT gDNA background of 10,000 copies/PCR to reach theoretical MAF of 5%, 2.5%, 1%, 0.5%, 0.25%, 0.1% and 0.05%. The PIK3CA Assay n°1 mixes included the four MUT gBlocks of the N345K-C420R-H1047L/R detection and the eight most frequent mutations identified on codons 542–546 (MAF: mutant allelic frequency).

Back to article page