Figure 3
Postsynaptic NMJ morphology is not affected by lithium treatment. LAL muscles from control- or lithium-treated groups were subjected to the two-colour BTX assay. Images from BTX1 and BTX2 staining from lithium- and control-treated muscle samples were subjected to a systematic approach to perform morphometric analyses at the NMJ to determine postsynaptic parameters including endplate diameter (A), perimeter (B), and area (C). We also determined nAChRs morphological parameters, such as nAChR area (D), perimeter (E), and compactness (F). Graphs represent the mean values ± SD along with individual data from 30 to 40 NMJs per animal (N = 3 animals per group). t-test were performed for pre-existing (BTX1) or new (BTX2) nAChRs, respectively. *p < 0.05.
