Figure 4 | Scientific Reports

Figure 4

From: The RhoA dependent anti-metastatic function of RKIP in breast cancer

Figure 4

RKIP regulates E-cadherin (E-cad) and subsequent cell invasion through ERK2. (a) Representative western blots with lysates prepared from control knockdown (shLUC) or RKIP knockdown (shRKIP) BT20 as indicated. Numbers are shown for quantified phosphorylated ERK1 or ERK2 bands normalized with total ERK protein. (b) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), ERK1 (shERK1) or ERK2 (shERK2) BT20 cells. (Bottom left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (c) (Left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, RKIP knockdown (shRKIP) or ERK2 knockdown (shERK2). right) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (d) (Left panel) Representative results of invasion assays with same set of cells used in (c) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. Unpaired Student's t-test (two-tailed) was used for all analyses with p < 0.05 considered significant. ns not significant.

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