Figure 6
From: The RhoA dependent anti-metastatic function of RKIP in breast cancer

Increased RhoA expression is required for RKIP-mediated suppression of breast cancer metastasis in a murine allograft model. (a) (Left panel) Representative western blots of lysates prepared from control knockdown (shLUC), or RKIP knockdown (shRKIP369) 4T1 cells. (Middle panel) Weight of primary tumors harvested from Balb/c mice 30 days after orthotopical implantation with the indicated 4T1 cells shown in the left panel. (Right panel) Number of lung metastatic nodules recorded in Balb/c mice 30 days after orthotopical implantation with the indicated 4T1 cells shown in the left panel. n = 6. (b) Representative western blots of lysates prepared from control knockdown (shCTR), or three different RhoA knockdowns (shrhoA#32-34) 4T1 gfp-luc cells. The data shown in Fig. 6(b) was used previously in another article (Fig. 2d)20. The duplicated data was used to confirm the RhoA expression was successfully knocked down in a breast cancer cell line that was used in both publications. (c) (Left panel) Representative ex vivo color images of lungs harvested from mice orthotopically injected with the indicated 4T1 gfp-luc cells showed in (b), (Right panel) Quantification of lung metastatic nodules (mean ± SE) detected in lungs harvested from mice orthotopically injected with the indicated 4T1 gfp-luc cells shown in (b). n = 4. (d) (Top panel) Representative ex vivo BLI images of lungs harvested from mice orthotopically injected with the indicated 4T1 gfp-luc cells shown in (b), (Bottom panel) Photon flux quantification of (mean ± SE) BLI images shown in the top panel. n = 4. (e) (Left) Representative hematoxylin and eosin (H&E) stained cross-sections of lungs harvested from mice orthotopically injected with the indicated 4T1 gfp-luc cells showed in (b) showing metastases demarcated from normal tissues with black solid line. (Right panel) total tumor metastases area calculated from the H&E sections shown above. n = 4. Unpaired Student's t-test (two-tailed) was used for all analyses with p < 0.05 considered significant. ns not significant.