Figure 7
From: The RhoA dependent anti-metastatic function of RKIP in breast cancer

RKIP suppresses breast cancer metastasis in part through downstream RhoA-regulated mechanisms. (a) (Right panel) Representative immunohistochemical (IHC) E-cad Ab staining images of breast primary tumor sections of mice orthotopically injected with control knockdown (shLUC) or RKIP (shRKIP) knockdown 4T1 gfp-luc cells. (Left panel). Quantification of areas per tumor field of view (FOV) stained positive in the right panel for E-cad. n = 4. (b) (Right panel) Representative immunohistochemical (IHC) E-cad Ab staining images of breast primary tumor sections of mice orthotopically injected with control knockdown (shCTR) or RhoA (shRhoA#32) knockdown 4T1 gfp-luc cells. (Left panel). Quantification of areas per tumor field of view (FOV) stained positive in the right panel for E-cad. n = 4. (c) (Right panel) Representative immunohistochemical (IHC) E-cad Ab staining images of breast primary tumor sections of mice orthotopically injected with 4T1 gfp-luc cells with different combinations of control knockdown, RhoA knockdown (shRhoA#34), empty vector control (EVC) or RKIP expression. (Left panel). Quantification of areas per tumor field of view (FOV) stained positive in the right panel for E-cad. n = 4. (d) (Right panel) Representative immunohistochemical (IHC) CCL5 Ab staining images of breast primary tumor sections of mice orthotopically injected with control knockdown (shLUC) or RKIP (shRKIP) knockdown 4T1 gfp-luc cells. (Left panel). Quantification of areas per tumor field of view (FOV) stained positive in the right panel for CCL5. n = 4. (e) (Right panel) Representative immunohistochemical (IHC) CCL5 Ab staining images of breast primary tumor sections of mice orthotopically injected with 4T1 gfp-luc cells with different combinations of control knockdown, RhoA knockdown (shRhoA#34), empty vector control (EVC) or RKIP expression. (Left panel). Quantification of areas per tumor field of view (FOV) stained positive in the right panel for CCL5. n = 4. (f) (Right panel) Representative immunohistochemical (IHC) F4/80 Ab staining images of breast primary tumor sections of mice orthotopically injected with control knockdown (shLUC) or RKIP (shRKIP) knockdown 4T1 gfp-luc cells. (Left panel). Quantification of areas per tumor field of view (FOV) stained positive in the right panel for F4/80. n = 4. (g) (Right panel) Representative immunohistochemical (IHC) F4/80 Ab staining images of breast primary tumor sections of mice orthotopically injected with 4T1 gfp-luc cells with different combinations of control knockdown, RhoA knockdown (shRhoA#34), empty vector control (EVC) or RKIP expression. (Left panel). Quantification of areas per tumor field of view (FOV) stained positive in the right panel for F4/80. n = 4. Unpaired Student's t-test (two-tailed) was used for all analyses with p < 0.05 considered significant. (h) A schematic depicts the mechanism through which RKIP activates E-cad to suppress cancer cells invasion.