Figure 3
From: LSD-stimulated behaviors in mice require β-arrestin 2 but not β-arrestin 1
Effects of LSD and MDL100907 on behavioral responses in β-arrestin 1 mice. A description of the experimental design is shown in the Fig. 1 legend. The head twitch, grooming, and retrograde walking results represent the first 30 min after injection of LSD in the open field. Nose poking was examined in a 5-choice serial reaction time apparatus (no rewards) with a similar time-course for the vehicle and MDL injections as in the open field, followed by administration of the vehicle and LSD. (a) LSD-stimulated head twitches in WT and βArr1-KO mice. A two-way ANOVA revealed a significant treatment effect [F(4,93) = 114.447, p < 0.001]. (b) LSD-stimulated grooming in βArr1 animals. A two-way ANOVA identified a significant treatment effect [F(4,93) = 61.232, p < 0.001]. (c) LSD-stimulated retrograde walking in βArr1 subjects. A two-way ANOVA found the main effect of treatment to be significant [F(4,93) = 43.899, p < 0.001]. (d) LSD-stimulated nose poking in WT and βArr1-KO mice. A two-way ANOVA observed a significant treatment effect [F(4,89) = 60.656, p < 0.001]. N = 8–17 mice/group for head twitch, grooming, and retrograde walking; N = 9–13 mice/group for nose-poking. Bonferroni corrected post-hoc tests for head twitch, grooming, retrograde walking, and nose-poking responses for treatment effects: p < 0.001, LSD vs. all groups; for retrograde walking for treatment effects: p < 0.05, 0.1MDL + LSD vs. all groups.
