Figure 4

Effects of LSD and MDL100907 on behavioral responses in β-arrestin 2 mice. A description of the experimental design is presented in the Fig. 3 legend. (a) LSD-stimulated head twitch responses in WT and βArr2-KO mice. A two-way ANOVA reported significant genotype [F(1,96) = 31.271, p < 0.001] and treatment effects [F(5,96) = 41.567, p < 0.001]; the genotype by treatment interaction was also significant [F(5,96) = 7.734, p < 0.001]. (b) LSD-stimulated grooming in βArr2 animals. A two-way ANOVA demonstrated significant genotype [F(1,96) = 51.972, p < 0.001] and treatment effects [F(5,96) = 27.987, p < 0.001]; the genotype by treatment interaction was also significant [F(5,96) = 7.953, p < 0.001]. (c) LSD-stimulated retrograde walking in βArr2 subjects. A two-way ANOVA found significant treatment effects [F(5,96) = 13.028, p < 0.001]; the genotype by treatment interaction was also significant [F(5,96) = 5.199, p < 0.001]. (d) LSD-stimulated nose poking in WT and βArr2-KO mice. A two-way ANOVA observed significant treatment effects [F(5,125) = 7.512, p < 0.001]; the genotype by treatment interaction was also significant [F(5,125) = 4.769, p = 0.001]. N = 8–12 mice/group for head twitch, grooming, and retrograde walking; N = 10–15 mice/group for nose-poking. *p < 0.05, ***p ≤ 0.001, WT vs. KO; ⁺p < 0.05, ⁺⁺p < 0.01, ⁺⁺⁺p ≤ 0.001, LSD vs. indicated groups within genotype; ^{^^}p < 0.01, ^{^^^}p < 0.001, 0.05MDL + LSD vs. indicated groups within genotype; ^††p < 0.01, ^†††p < 0.001, 0.1MDL + LSD vs. indicated groups within genotype.