Figure 1
From: Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
PrPres detected in PMCA reactions seeded with brain material (parietal cortex) from atypical scrapie inoculated pigs. Positive PMCA reactions show an identical glycoform pattern to that of cattle C-BSE prions and/or cattle C-BSE propagated in tgBov substrate (third line). Positive reactions were obtained from pigs inoculated with both original atypical scrapie isolates (PS152 isolate: P-1215 and P-1216; TOA3 isolate: P-1228). PMCA reactions seeded with brain homogenate from a TSE negative sheep and unseeded PMCA reactions are included as controls of the technique. PMCA reactions were subjected to 3 amplification rounds (15 s sonication, 14 min 50 s incubation at 39 °C) in a Qsonica700 sonicator and analyzed for PrPres by western blot using the monoclonal anti-PrP antibodies Sha31 and 12B2. WB control = classical scrapie isolate.
