Figure 2

Validation of RNA-Seq data using ABI Quant studio qPCR system. The mean expression of ten selected genes was calculated by − ΔΔCT method and normalised by mean of Ct values of YWHAZ and TBP YWHAZ as reference genes. The values were converted into log2 fold change (LFC). Each dot point represents one gene. Pearson correlation coefficient test used to compare the results and its value labelled as “R”. Plus (+) and minus (−) signs indicate log2 FC values for the upregulated and downregulated genes, respectively.