Figure 5 | Scientific Reports

Figure 5

From: Phytosterol accumulation results in ventricular arrhythmia, impaired cardiac function and death in mice

Figure 5

RNA-seq analysis of heart samples. (A) Plot of first two components of principal component analysis (PCA) of gene expression. ABCG5/8 double knockout (DKO) samples are filled with red and ABCG5/8 NPC1L1 triple knockout (TKO) samples are filled with cyan. Vehicle samples are triangles and ezetimibe treated samples are circles. (B) Volcano plots of P values from differential expression analysis versus log2 fold change in gene expression as calculated using DEseq2 comparing DKO untreated to TKO or DKO ezetimibe treated samples. A positive fold change value indicates higher expression in ABCG5/8 DKO untreated samples. (C) Venn diagram demonstrating overlap in differentially expressed (DE) genes from two comparisons. (D) Heatmap of gene expression (quantile normalized FPKM) Z-scores of DE genes identified comparing DKO and TKO conditions. DKO + vehicle: N = 6/group; DKO + ezetimibe: N = 5/group; TKO + vehicle: N = 5/group; TKO + ezetimibe: N = 5/group.

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