Table 1 Worsening histological tubular injury and inflammation with increasing warm ischemia. A Histological scores of tubular injury (0–3) and inflammation (0–3) assessed by a renal pathologist blinded to the groups. Scores taken at time of sacrifice on post-operative day 7 between all groups. Statistical difference p < 0.05 with $: HBD vs DCD60min, *:HBD vs DCD120min, #:DCD30min vs DCD120min, In brackets: p < 0.01. HBD Heart beating donor. DCD Donation after cardiac death. B Tubular injury and C inflammatory semiquantitative scores were determined using these parameters.
A | POD7 | |
|---|---|---|
Median Tubular Injury Score (Range) | Median Inflammation Score (Range) | |
HBD | 0.5 (0.0–0.5) | 0.5 (0.5–1.0) |
DCD30MIN | 0.5 (0.5–1.0) | 0.5 (0.0–1.5) |
DCD60MIN | 1.0 (1.0–2.0)(*)(#) | 0.5 (0.5–1.0) |
DCD90MIN | 2.0 (1.5–3.0)(*)(#) | 1.0 (1.0–1.5) |
DCD120MIN | 3.0 (3.0–3.0)(*)(#)($) | 2.0 (1.0–2.0)*#$ |
B. Tubular Injury Score | C. Inflammation Score | |
|---|---|---|
Criteria | Brush border loss | Number of infiltrating interstitial mononuclear cells averaged over 30 high powered fields |
Tubular dilatation | ||
Epithelial vacuolation | ||
Thinning and sloughing | ||
Luminal debris |
