Figure 4
From: Dectin-2 mediates phagocytosis of Lactobacillus paracasei KW3110 and IL-10 production by macrophages
Inhibition of Dectin-2-syk pathway abolishes the effect of KW3110 on IL-10 production. (a, b) Dectin-2 knockout RAW 264.7 cells were treated with KW3110 for 24 h. (a) IL-10 concentration in the supernatants was measured by ELISA. (b) The amount of incorporated KW was measured by flow cytometry. (c, d) RAW 264.7 cells were treated with KW3110 in the presence or absence of anti-Dectin-2 antibody for 24 h. (c) IL-10 concentration in the supernatants was measured by ELISA. (d) The amount of incorporated KW was measured by flow cytometry. (e, f) Syk knockout RAW 264.7 cells were treated with KW3110 for 24 h. (e) IL-10 concentration in the supernatants was measured by ELISA. (f) The amount of incorporated KW was measured by flow cytometry Significant differences were compared to the control group, *p < 0.05, **p < 0.01. Data are the means ± SD. Data are representative of at least three independent experiments.
