Figure 3
From: ApoE4 attenuates autophagy via FoxO3a repression in the brain
Autophagy/mitophagy components were quantitatively altered in the brains of ApoE4 carriers. The protein levels of p62, LC3, p-ubiquitin (Ser65), optineurin, and β-actin in ApoE4 non-carriers (n = 7, E4-) vs. carriers (n = 5, E4 +) were analyzed by immunoblotting using corresponding antibodies, respectively. Representative western blot analysis of p62 and LC3 (A), p-ubiquitin (Ser65), and optineurin (B). β-actin was used as a loading control. Full blots are provided in Supplementary Fig. S4. Relative ratio of p62 (C), LC3-II (D) p-ubiquitin (Ser65) (E), and optineurin (F) against the protein levels indicated on the y-axis, respectively. Data shown are mean ± SEM. (**p < 0.01). ns not significant.
