Figure. 4

JQHF reduce the apoptosis in the kidneys of PHN rats. (a,e) Western blot analysis of the expression of Cyt c, Cleaved caspase-3 and Nephrin protein in each group. Full-length blots and all replicates performed are presented in Supplementary Figs. 2, 3. (b,f) Quantitative analysis of the expression of Cyt c, Cleaved caspase-3 and Nephrin protein. (c) Determination of apoptosis rate by Annevin-V-FITC staining. (d) Quantifcation of the apoptosis rate in each group. (g) Immunohistochemistry staining of Nephrin protein (original magnifcation ×400). (h) Analysis of IOD of Nephrin. Data were analysed by one-way ANOVA followed by post hoc LSD test (b,f,h) and presented as the mean ± SD (n = 6). Data were analysed by Kruskal Wallis H test (d) (n = 6). *P < 0.05 vs. Normal group, **P < 0.01 vs. Normal group, ^#P < 0.05 vs. Model group, ^##P < 0.01 vs. Model group, ^○P < 0.05 vs. Benazepril group, ^○○P < 0.01 vs. Benazepril group and ^□□P < 0.01 vs. TET group.