Table 6 Sequences Of the primers used for PCR-based characterization of the transgenic lines and the resulting product sizes.

From: Heterologous production of hyaluronic acid in Nicotiana tabacum hairy roots expressing a human hyaluronan synthase 2

Primers

Sequence

Product length (bp)

Ta (˚C)

RolB-F

AAGTGCTGAAGGAACAATC

194

54

RolB-R

CAAGTGAATGAACAAGGAAC

VirG-F

CCTTGGGCGTCGTCATAC

529

55

VirG-R

TCGTCCTCGGTCGTTTCC

hHAS2-F

AACATCTGGGCGAAAAACC

203

58.7

hHAS2-R

CGACATAAAGCGTAAGGAGCA

  1. The primers were designed using Vector NTI 11 and Allele ID 7 software.
  2. Ta temperature annealing, F forward, R reverse.
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