Figure 5
Validation of synapsin-1 (Syn1), α-tubulin, cytochrome c (Cyt c), HSP90, glutamate dehydrogenase 1 (Glud1) and glutamine synthetase (Glul) in synaptosomal fractions of rat hippocampus from Controls, Control + Tian, CSIS and CSIS + Tian groups by Immunohistochemical (a) and Western blot analysis (b–f). Data were expressed as mean ± SEM, n = 4–6 independent measurements in each group. Significant differences between groups obtained from two-way ANOVA analyses followed by Duncan’s post-hoc test are indicated as follows: *p < 0.05, **p < 0.01, comparisons always against Controls; ^p < 0.05, CSIS + Tian vs. CSIS. Displayed blots are cropped images of representative examples of several Western blots performed. Full-length blots are presented in Supplementary Figure S1. The missing parts of the membranes are due to unsatisfactory antibody hybridization, and thus were not represented or used in the final analysis.
